Additional Selective and Differential Cell Culture Media
NEOGEN Malt Extract, 500 g
Malt Extract is a dehydrated extract of malt for use in preparing microbiological culture media in a laboratory setting. Malt Extract is not intended for use in the diagnosis of disease or other conditions in humans.Malt Extract is a clarified, water-soluble extract of malted barley. Malt Extract is a useful ingredient of culture media designed for the propagation of yeasts and molds. This ingredient is suitable for yeasts and molds because it contains a high concentration of carbohydrates, particularly maltose. The approximate percentage of reducing sugars in Malt Extract is 60 – 63%.The high carbohydrate content makes malt extract very sensitive to overheating which results in darkening of the medium it is used in. Malt Extract is generally employed in culture media at concentrations between 10 to 100 grams per liter.For more information visit https://www.neogen.com/solutions/Microbiology/malt-extract
NEOGEN Agar, Bacteriological No. 2, 10 kg
Agar, Bacteriological No. 2 is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Bacteriological No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperature for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.For more information visit https://www.neogen.com/solutions/Microbiology/agar-bacteriological-number-2
NEOGEN DRBC Agar (ISO), 5 kg
Dichloran Rose Bengal Chloramphenicol Agar is for the enumeration of yeasts and is not intended for use in the diagnosis of disease or other conditions in humans.Developed with reference to ISO 21527-1:2008 for the enumeration of yeasts and molds in food and animal products, this medium is tested to the performance requirements of the standard. Used for the enumeration of viable yeasts and molds in products with a water activity of greater than 0.95 such as eggs, meat, some dairy products, fresh pastes, fruit and vegetables, DRBC Agar is designed to suppress the colonial growth of ‘spreader’ molds and in doing so allow easier performance of the colony count technique on yeasts and molds.For more information visit https://www.neogen.com/solutions/Microbiology/drbc-agar-iso
NEOGEN Yeast & Mold Agar, 500 g
Yeast and Mold Agar is recommended for the isolation and maintenance of yeasts and molds. This medium can also be used for the detection of wild yeasts in beer. Yeast and Mold Agar is not intended for use in the diagnosis of disease or other conditions in humans. Yeast and Mold Agar is prepared according to the formulation published by Wickerham and was formulated for the selective isolation of yeasts from mixed cultures containing bacteria and molds. The selectivity of this medium may be enhanced through acidification or through addition of selective agents. Yeast and Mold Agar should be sterilized without pH adjustment and sterile acid added to the medium cooled to 45 - 50°C. Acidified Yeast and Mold Agar should not be heated. Antibiotics may be aseptically added to sterile media.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-and-mold-agar
NEOGEN MacConkey Agar No. 2, 500 g
MacConkey Agar No.2 is a modification of MacConkey Agar which contains bile salts No. 2 for the recognition of Enterococci, and is not intended for use in the diagnosis of disease or other conditions in humans.This agar especially useful when looking for Enterococci in the presence of coliforms and non-lactose fermenters from water, sewage and food products. Enterococci are frequently sought as an index of fecal pollution and appear on this medium as small, intensely colored red-purple colonies. Non-lactose fermenters appear colorless, whilst bile tolerant Gram-positive organisms, such as staphylococci and non-fecal streptococci, are completely inhibited.Formula:Peptone - 20.0 g/LLactose - 10.0 g/LBile Salts No. 2 - 1.5 g/LSodium Chloride - 5.0 g/LNeutral Red - 0.05 g/LCrystal Violet - 0.001 g/LAgar - 15.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/macconkey-agar-number-2
NEOGEN TAT Broth, 10 kg
TAT Broth is used for the detection of microorganisms in cosmetics and topical drugs in a laboratory setting. TAT Broth is not intended for use in the diagnosis of disease or other conditions in humans. TAT Broth is prepared for use in the examination of cosmetics and topical drugs. TAT Broth, with the addition of Polysorbate 20, is recommended for sterility testing of viscous materials, including salves or ointments. It is especially adapted for sterility testing of cosmetics. Cosmetic products are subject to contamination during manufacturing and use by consumers. Preservatives are used in aqueous products to make them self-sterilizing for vegetative bacteria, yeast, and molds. TAT (Tryptone-Azolectin-Tween) Broth is also referred to as Fluid Casein Digest-Soy Lecithin Polysorbate 20 Medium.For more information visit https://www.neogen.com/solutions/Microbiology/tat-broth
NEOGEN MacConkey Agar without Crystal Violet, 5 kg
MacConkey Agar without Crystal Violet is used for the isolation and differentiation of Gram-negative enteric bacilli and is not intended for use in the diagnosis of disease or other conditions in humans.A selective medium for the isolation of bile tolerant organisms from sewage and foodstuffs. Bile-tolerant Gram positive organisms as well as Gram negative organisms will grow on this medium. This formula is recommended by W.H.O. and other bodies for the examination of water and milk. Some strains of Proteus spp. will spread on this medium making interpretation difficult, for this reason MacConkey Agar (without salt) may be preferred as it is less prone to this phenomenon.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-agar-without-crystal-violet
NEOGEN LEE Broth, 500 g
Listeria Express Enrichment Broth (LEE Broth) is a selective enrichment broth for the detection of Listeria from foods and is not intended for use in the diagnosis of disease or other conditions in humans.Developed to give improved growth rates of Listeria over traditional selective enrichment media, LEE Broth enhances the expression of target antigens for most commercially available immunological test kits/methods whilst maintaining adequate suppression of potential non-target organisms. Selective components are blended into the powder, removing the requirement for supplementation.Compared with more traditional methods, immunological tests, such as ELISA, require relatively high levels of target organisms to achieve a reliable positive result. LEE Broth has been specifically designed to stimulate growth from low numbers to the required high levels within a 24 hour period.For more information visit https://www.neogen.com/solutions/Microbiology/lee-broth
NEOGEN Raka-Ray No. 3 Agar, 5 kg
Raka-Ray No.3 Agar is for the detection of lactic acid bacteria in beer and for monitoring in-process beer quality, and is not intended for use in the diagnosis of disease or other conditions in humans. It is recommended for this application by the European Brewing Convention (EBC) and the American Society of Brewing Chemists (ASBC).Contamination of beer and the beer making process by members of the lactobacilli family results in spoilage, primarily through their production of metabolic products which are detrimental to the flavor of the final product. Detection of these organisms is complicated by their diverse nutritional and environmental requirements.A number of different formulations have been described for the isolation of lactic acid bacteria in brewing products and processes. Raka-Ray Agar was developed by the addition of various growth promoting compounds to Universal Beer Agar.For more information visit https://www.neogen.com/solutions/Microbiology/raka-ray-3-agar
NEOGEN Campy Cefex Agar, 500 g
Campy Cefex Agar is used for the selective isolation of Campylobacter spp in a laboratory setting. Campy Cefex Agar is not intended for use in the diagnosis of disease or other conditions in humans.Poultry is a primary reservoir of Campylobacter spp. and may cause contamination of more than 80% of chicken carcasses. Campy Cefex Agar is described by Stern et al. This medium was formulated to provide selective isolation of cephalothin-resistant Campylobacter species such as C. jejuni and C. coli.For more information visit https://www.neogen.com/solutions/Microbiology/campy-cefex-agar
NEOGEN DC Medium with BCIG, 5 kg
DC Medium with BCIG is used in the chromogenic differentiation of E. coli from other coliforms in water samples using the membrane filtration method in a laboratory setting. DC Medium with BCIG is not intended for use in the diagnosis of disease or other conditions in humans.DC (Differential Coliform) Medium with BCIG is a selective and differential medium for the presumptive identification of E. coli. This medium is enhanced by the addition of a chromogenic agent, BCIG, 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide to detect glucuronidase activity. The presence of the enzyme ß-D-glucuronidase differentiates most E. coli spp. from other coliforms, and is the same enzyme used in the MUG reaction. BCIG reacts slightly differently, and when released into the medium is insoluble, accumulating within the cell, imparting a blue to purple color to presumptive E. coli colonies.For more information visit https://www.neogen.com/solutions/Microbiology/dc-medium-with-bcig
NEOGEN Urea Agar Base (Christensen), 500 g
Urea Agar Base is used with agar for the differentiation of microorganisms on the basis of urease production in a laboratory setting. Urea Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Christensen devised a urea agar medium containing peptone and dextrose that had a reduced buffer content. The medium supported the vigorous growth of many Gram-negative, enteric bacilli and readily permitted observation of urease production. Urea Agar Base may be used as a screening medium (along with Triple Sugar Iron Agar) for the selection of Salmonella and Shigella cultures for serologic classification. Urea Agar Base is also used to detect production of urease by yeast. Urease production is an important differential test in microbiology and outlined in standard methods.For more information visit https://www.neogen.com/solutions/Microbiology/urea-agar-base-christensen
NEOGEN Tryptic Soy Broth, Modified with 8 mg Novobiocin & Acid Digest Casein, 500 g
Tryptic Soy Broth, Modified with Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods. Conforms to USDA Formulation with Novobiocin supplementation in a laboratory setting. Tryptic Soy Broth, Modified with Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. Tryptic Soy Broth, Modified with Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth-modified-with-8-mg-novobiocin-and-acid-digest-casein
NEOGEN Modified Buffered Peptone Water with Pyruvate, 10 kg
Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans. Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables. Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-buffered-peptone-water-with-pyruvate
NEOGEN Palcam Agar Base, 5 kg
Palcam Agar Base is used with supplements as a selective and differential medium for the detection and isolation of Listeria monocytogenes from food and environmental samples. Palcam Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Palcam Agar was developed by Van Netten et al in 1989 as an improved selective differential medium for the isolation of Listeria monocytogenes from food and environmental specimens. Improved selectivity is achieved by the combination of antibiotic supplements and microaerobic incubation. The double indicator system of esculin hydrolysis and mannitol fermentation aids differentiation of Listeria spp from enterococci and staphylococci which can be confused with Listeria spp on other types of culture media. Palcam Agar is listed within Annex E of ISO 11290-1:2017 as a suitable second selective agar.For more information visit https://www.neogen.com/solutions/Microbiology/palcam-agar-base